Liquid culture: phase 1

As already said, liquid culture consists of mycelium grown in a solution of water and nutrients, which can be of various kinds (to begin we will use the honey). As containers, we will use normal cans, like those we saw in the previous post. However, you must make a fix to the cap: we'll need to add a filter to allow gas exchange, and create an injection port site that will allow us to inoculate jars and vacuum the mycelium at the right time. The process is very simple.

We take our beautiful, brand new jar:

 

Remove the lid and (with a drill, a dremel, a screwdriver used as a chisel or whatever you want) make two holes on either side of the can, a smaller and a larger one (beware not to pierce the thread!).



We apply a bit of silicone, both above and below the smaller hole, shaping it so that it remains a bit in relief on both sides:






Once the silicone has dried (it takes a few hours) make a scoop of polyfill and get it stuck in force in the other bigger hole:







 Well, our cap is ready. So we can take to the next step, which I'll explain in the next post;)